Examination of the Qβ-Replicase Reaction by Sucrose Gradient and Gel Electrophoresis
نویسندگان
چکیده
منابع مشابه
Bacterial analysis of combined periodontal-endodontic lesions by polymerase chain reaction-denaturing gradient gel electrophoresis.
We used denaturing gradient gel electrophoresis (DGGE) to compare bacterial profiles in periodontium and root canals of teeth with combined periodontal-endodontic lesions. Samples of dental plaque and necrotic pulp were collected from thirteen extracted teeth with advanced periodontitis. Genomic DNA was extracted for polymerase chain reaction (PCR) analysis using universal bacterial primers. Th...
متن کاملCharacterization of wine yeasts by temperature gradient gel electrophoresis (TGGE).
18S rDNA from 74 wine yeast strains was amplified by PCR using specific primers, and the products analyzed by temperature gradient gel electrophoresis (TGGE). TGGE is a useful method in screening the genotypes of the wine yeasts. Intraspecific differentiation was achieved on the basis of TGGE in some cases, whereas in others identical bands for strains classified as separate species were obtain...
متن کاملGradient gel electrophoresis of human plasma lipoproteins.
A rapid and simple method for separating and identifying plasma lipoproteins is described. The technique utilizes the “molecular sieving” properties of continuous concave gradient electrophoresis on a slab of polyacrylamide (4 to 26%) gel. Gradient gel electrophoresis is particularly suitable for separating lipoproteins according to their molecular weight. The technique can be used to demonstra...
متن کاملFractionation of heparin-derived oligosaccharides by gradient polyacrylamide-gel electrophoresis.
Heparin-derived oligosaccharides, prepared by using flavobacterial heparinase, having a high degree of heterogeneity (sequence variability) were resolved into sharp well-defined bands by using polyacrylamide gel electrophoresis (PAGE). The use of a stacking gel and a high-density-pore-gradient resolving gel was primarily responsible for the success of this separation. Low-Mr standards of known ...
متن کاملcomparative analysis of denaturing gradient gel electrophoresis and temporal temperature gradient gel electrophoresis profiles as a tool for the differentiation of candida species
results the results showed that nl1-gc/ls2 primer set could yield species-specific amplicons, which were well distinguished and allowed better species discrimination than that generated by the its3-gc/its4 primer set, in both dgge and ttge profiles. all five candida species were discriminated by dgge and ttge using the nl1-gc/ls2 primer set. conclusions comparison of dgge and ttge profiles obta...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Virology
سال: 1967
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.1.4.771-778.1967